Search results for "Protein content"
showing 10 items of 16 documents
Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E
2021
ABSTRACTThe ARF family of regulatory GTPases is ancient, with 16 members predicted to have been present in the last eukaryotic common ancestor. Our phylogenetic profiling of paralogs in diverse species identified four family members whose presence correlates with that of a cilium/flagellum: ARL3, ARL6, ARL13, and ARL16. No prior evidence links ARL16 to cilia or other cell functions, despite its presence throughout eukaryotes. Deletion of ARL16 in MEFs results in decreased ciliogenesis yet increased ciliary length. We also found Arl16 KO in MEFs to alter ciliary protein content, including loss of ARL13B, ARL3, INPP5E, and the IFT-A core component IFT140. Instead, both INPP5E and IFT140 accum…
Protein and β-ODAP content and their association with yield contributing traits in selected grass pea lines
2009
Eight selected grass pea (Lathyrus sativus L.) lines were evaluated over three different growing seasons (2005-2008) at the experimental field at the ‘Sparacia farm’ (Cammarata-AG) in Sicily. A randomized complete-block design with three replicates was adopted. 35 viable seeds/m2 of each line were placed at the end of autumn in rows distant 50 cm. After one summer ploughing, 90 kg ha-1 di P2O5 were filled during two harrowing before sowing; later two manual weeds control were performed. Harvest was executed at full maturity of pods (end of the spring). Temperature values and effective rainfall for each season were recorded. Information are presented on the variability in storage seed protei…
The Impact of Copper Ions on Oxidative Stress in Garden Cress Lepidium sativum
2017
Abstract Normal oxygen metabolism is an endogenous source of reactive oxygen species (ROS). The source of ROS are also many environmental factors including heavy metals. In certain concentration range, the presence of ROS is necessary to maintain proper cell function. Thus, cells have many mechanisms, which role is focused on maintaining a constant concentration of ROS. Imbalance between the formation of ROS and action of a protective antioxidant system leads to oxidative stress. This may results with a damage to the structure of proteins, lipids and nucleic acids, which in turn can lead to disturbances in the functioning of the cell and even to the death. The aim of the study was to evalua…
Diet high in protein-rich foods with structured sport activity may be useless to lose fat mass and maintain fat-free mass
2020
Background The aim of this study was to demonstrate that a normal protein diet along with minimal sports activity can be enough to lose fat mass and maintain muscle mass. Methods All participants were prescribed a hypocaloric nutritionally balanced Mediterranean-style diet tailored to the individual for 8 weeks. Body composition and energy expenditure were measured. Sedentary patients (G1) were only recommended to perform minimal aerobic training, while sport subjects (G2) were prescribed structured physical activity and higher calorie and protein contents in the diet. Results There were no significant differences between the two groups for any of the measured parameters. Conclusions The mo…
Protein content and lipid profiling of isolated native autophagosomes
2021
AbstractAutophagy is a central eukaryotic catabolic pathway responsible for clearance and recycling of an extensive portfolio of cargoes, which are packed in vesicles, called autophagosomes, and are delivered to lysosomes for degradation. Besides basal autophagy, which constantly degrades cellular material, the pathway is highly responsive to several stress conditions. However, the exact protein content and phospholipid composition of autophagosomes under changing autophagy conditions remain elusive so far. Here, we introduce a FACS-based approach for isolation of native unmanipulated autophagosomes and ensure the quality of the preparations. Employing quantitative proteomics and phospholip…
Controlled temperature grinding under modified atmosphere for Almond (Prunus Dulcis) paste production
2011
The quality of the raw material and the processing conditions have a great influence on the nutritional properties of finished products. This research is focused on almond paste production and aims at developing a new production process to deliver the high nutritional content of the raw almonds into the finished product. Raw almonds are composed mainly of fats (approx. 50%) and proteins (approx. 25%), and are particularly rich in omega 6 fatty acids, and are hence exposed to denaturation and oxidation phenomena during the traditional grinding/homogenization process. The study involved the analysis of six different local cultivars based upon the main nutritional indicators (protein content, …
Physiological and oral parameters contribute prediction of retronasal aroma release in an elderly cohort.
2021
International audience; Malnutrition is a serious problem in the elderly while understanding flavour perception could be a tool for controlling appetite or food choices. To increase our knowledge, we characterised the health and oral physiology (oral volume, swallowing tongue force, number of teeth and salivary flow rate, protein content and antioxidant capacity) of a cohort of 54 community-dwelling French elderly as well as their individual retronasal release of five aroma compounds (2-pentanone, 2-nonanone, 2,3-hexanedione, octanal and linalool) by proton-transfer-reaction mass spectrometry (PTR-MS). In general, large variability across participants was observed in both oral physiological…
In vivo digestibility and nutritive value of Atriplex halimus alone and mixed with wheat straw
2002
In vivo digestibility trials were carried out using six young rams fed with Atriplex halimus biomass harvested in summer (A) and in early autumn (B), and with a mixture of mid-autumn Atriplex halimus and wheat straw (5[ratio ]1 ratio on fresh matter basis) (C). Atriplex halimus had a high protein content (139·0, 135·9 and 193·4 g/kg DM in A, B and C respectively), but was rich in sodium chloride, especially in summer (145·9 g/kg DM), limiting its use as feed. The summer forage had a higher organic matter (OM) digestibility coefficient than the autumn forage (0·663 v. 0·530) but lower digestible OM intake (16·8 v. 29·4 g/day per kg BW0·75). In autumn forage, the combination with straw did no…
Influence of grain quality, semolinas and baker’s yeast on bread made from old landraces and modern genotypes of Sicilian durum wheat
2021
Abstract Several studies showed that products made with ancient wheat genotypes have beneficial health properties compared to those obtained with modern wheat varieties, even though the mechanisms responsible for the positive effects are not clear. Ancient durum wheat genotypes are being currently used for the production of pasta, bread and other typical bakery products but the consumption is strictly local. In this work 15 genotypes of Triticum turgidum subsp. durum, including 10 ancient and 5 modern, were characterized for their technological traits through the determination of different parameters: protein content, dry gluten, gluten index, yellow index, ash, P/L, W and G. In addition, t…
Fruit production and quality evaluation of four litchi cultivars (Litchi chinensis Sonn.) grown in Mediterranean climate
2017
Introduction - The agronomic and qualitative responses of the litchi fruit (Litchi chinensis Sonn.) grown in Mediterranean climate are not yet studied. In this study, yield components, physico-chemical and sensory traits of four commercial litchi cultivars were recorded over two productive seasons. Materials and methods - Fruits of the cvs Tai So, Wai Chee, Brewster and Kwai Mai were collected at commercial maturity stage and subjected to productive (yield, efficiency, number of fruits, crop load), analytical (fruit weight, transversal diameter, longitudinal diameter, moisture, total soluble solid content (TSS), titratable acidity (TA), TSS/TA ratio, seed weight, peel weight, percentage of …